Consistently with the preleukemic HSPCs in vitro, sgCas9 completely depleted the expression of Cas9 in the resulting sgSsty1-sgCas9; AML1-ETO and sgSsty2-sgCas9; AML1-ETO AML cells (Supplemental Figure 4D). Here, RUNX1T1 is linked to acute myeloid leukemia.