Simultaneously, following infection with HD-13 for 0 h, 1 h, 6 h, and 12 h, respectively, in 3D4/21, Western blot was carried out to detect the levels of NF-κB pathway-related proteins, including p65, p-p65, IKKα, and p-IKKα. The gene discussed is NFKB1; the disease is infection.