IFNG and neoplasm: In this regard, IFN-γ is often used in combination with chemotherapy.242 In early research, an anti-TAG72 scFv-IFN-γ fusion protein exhibited the antigen-binding specificity of scFv moiety and cytokine activity in vitro.243 L19 scFv against the ED-B domain of fibronectin was fused to the N-terminus of a cysteine-free mutant of murine IFN-γ to generate an anti-ED-B-scFv-IFN-γ fusion protein.242 The fusion protein targeted blood vessels in solid tumors, and the targeting efficiency was strikingly increased in tumor‐bearing IFN‐γ receptor-knockout mice.