In the human breast cancer MCF-7 cell line, metformin decreases phosphorylation of cofilin, an actin-binding protein involved in cytoskeleton dynamics, which is upstream regulated by LIM-kinase and Rho-kinase, suggesting a possible interaction of metformin with Rho-kinase (Özdemİr and Ark, 2021). This evidence concerns the gene CFL1 and breast carcinoma.