The upregulated genes were enriched in cell cycle-related pathways such as “Meiosis,” “Meiotic recombination,” “Cell cycle” as well as DNA repair pathways such as “double-strand break repair” and “ATM pathway.” These results demonstrate that HNRNPU knockout could rewire the transcriptome of bladder cancer cells, resulting in increased vulnerability to cisplatin treatment. Here, HNRNPU is linked to urinary bladder cancer.