We observed down-regulation of astrocytic network markers Cx43 and Cx30 in CSDS exposed mice, while treatment with EDA significantly increased the expression of Cx43 and Cx30 in the Hip and mPFC (Fig. 6).Together, these results suggested that the microglial and astrocytic abnormality in CSDS exposed mice can be restored by the replenishment of EDA, suggesting a potential therapeutic strategy for depression. Here, GJA1 is linked to depressive symptom measurement.