Based on these previous observations, we first set out to establish an infection model using murine WT and caspase-1/11−/− BMDMs inoculated with WT PA103, isogenic PA103 mutants encoding ExoU only (exoT::Tc) or ExoT only (ΔexoU) or lacking both ExoU and ExoT (ΔexoU exoT::Tc), or a T3SS mutant (ΔpcrV) (genotypes described in Table 1). Here, CASP1 is linked to infection.