Because transduced cells for T1 were derived from PBMCs collected during the chronic phase of infection, we performed in situ tetramer staining on lymph node biopsies, and flow cytometry on PBMCs collected after infusion to determine whether any of the CAR/CXCR5-T cells expressed the immunodominant Mamu A01 Gag/CM9 responsive TCR. Here, CXCR5 is linked to infection.