PYCR1 and neoplasm: rtPCR analysis revealed no increase in Pycr1 mRNA expression in intestinal tissue harvested from mice three days or one week after depletion of the tumor suppressor Apc, but accumulation of the Pycr1 transcript and protein in intestinal tissue became evident two weeks post Apc depletion (Figs 2A, 2B and S4).