TGFB1 and neoplasm: EMT was reported to play an important role in tumor development and metastasis.7 To test whether PFD treatment suppresses TGF-β-induced EMT, we first established the EMT model of renal cancer cells by testing gradient doses of TGF-β and found that 5 ng/ml of TGF-β was optimal for inducing EMT of ACHN and 786-O cells (data not shown).