The RT-qPCR results showed that the mRNA levels of epithelial markers E-cadherin and tight junction protein 1 (ZO-1) were increased, whereas the mRNA levels of mesenchymal markers N-cadherin and Vimentin were decreased after miR-149 upregulation (p < 0.0001) (Figure 2(e)), indicating that miR-149 diminishes EMT activity of ESCC cells. The gene discussed is CDH2; the disease is esophageal squamous cell carcinoma.