SDC4 and rhabdomyosarcoma: Because the activation of Rac1 GTPase increases myoblast fusion [12], Rac1 is necessary and sufficient for rhabdomyosarcoma cell migration and invasion [49], and syndecan-4 regulates Rac1 level [33, 34], we next analyzed the role of Rac1 in syndecan-4-dependent myoblast differentiation and fusion.