Besides this, HSP upregulated some intracellular ROS, ATP, Ca2+ and cytosolic components i,e AIF, Apaf-1, Cyt C, caspase-3, caspase-9, Bax, and down-regulated Bcl-2, mitochondrial AIF, mitochondrial Apaf-1, and mitochondrial Cyt C that mediate apoptosis of cancer cell [77]. Here, APAF1 is linked to cancer.