Recently, researchers examined the impact of HSP derivatives on liver cancer both in vivo and in vitro. They found that HSP derivative decreased the level of ALP, ALT, AST, TGF-β1, HA, Hyp, F4/80+ macrophage infiltration, MCP-1, TNF-α, IL-1β, IL-6, TNF-α, and IL-1β, Gli-1, Shh expression at 25–100 mg/kg concentration in Littermate male C57BL/6J mice [85]. This evidence concerns the gene SHH and liver cancer.