As a method to verify the key RNAs identified using transcriptome sequencing, we collected 20 pairs of clinical samples (20 bladder cancer tissue samples and 20 corresponding tumor-adjacent tissues, Supplementary Material S1), and performed qRT–PCR to detect the expression levels of MIR100HG, miR-142-5p and CALD1 in clinical tissues. Here, CALD1 is linked to urinary bladder cancer.