while AP3S1 and RPS15 had not been reported as driver genes before, and there were hotspot mutations in the AP3S1 (Figure 5F) and RPS15 (Figure 5G) in the ESCC, the mutation frequency of AP3S1 and RPS15 were low in the TCGA, and there was not hotspot mutation in the TCGA (Supplementary Figures S2, S3). The gene discussed is AP3S1; the disease is esophageal squamous cell carcinoma.