When the routine cut-off value of NSE is adopted, the positive rate of NSE in the serum of the control group is also high (42%), the cut-off value of NSE with the most diagnostic efficacy in ESCC is 17.5 ng/ml and the area of AUC is 0.64 (P < 0.05). Here, ENO2 is linked to esophageal squamous cell carcinoma.