The areas under the curves of CEA, CYFRA21-1 and NSE were 0.70, 0.71 and 0.64 respectively (all P < 0.05), the sensitivity was 80%, 88.89% and 60% respectively, and the specificity was 53%, 58.5% and 58% respectively, indicating that the above three tumor markers have obvious diagnostic values in esophageal carcinoma, especially CYFRA21-1. This evidence concerns the gene ENO2 and carcinoma of esophagus.