Then, we performed a technical validation of six candidate genes with MassARRAY EpiTYPER in CRC, followed by a comprehensive study to analyze the DLX6-AS1 methylation pattern at different stages of colorectal neoplasms, from nonadvanced adenoma (NAA) to advanced adenoma (AA) to colorectal carcinoma. This evidence concerns the gene DLX6 and colorectal neoplasm.