These findings strongly reminded that Sohlh2 might function through the following pathway: Sohlh2 inhibits the expression of DNMT3a, and the downregulation of DNMT3a reduces the methylation of Klotho, the reduction of Klotho methylation upregulates the expression of Klotho, and the upregulation of Klotho inhibits the proliferation, migration, invasion, and EMT of renal cancer cells (Figure 7). The gene discussed is SOHLH2; the disease is renal carcinoma.