The expression signatures at the mRNA level of the 8 genes were then investigated in four human BC cell lines (i.e. EJ, T24, UM-UC-3 and SW780) and demonstrated that the mRNA levels of CNKSR1 were relatively higher in most of the BC cells when compared with normal human urinary tract epithelial cell line (SV-HUC-1), however CXorf57 and FASN were dramatically decreased in most of the BC cells (Figs. S4A–S4H). This evidence concerns the gene FASN and breast cancer.