To determine whether Nodal affects the expression of neurotrophins (NGF, BDNF, and GDNF) and MMP-9 in pancreatic cancer cells, Panc-1 and BxPC-3 cells were treated with rhNodal (0, 50, and 100 μM) for 24 h. This evidence concerns the gene BDNF and familial pancreatic carcinoma.