Moreover, in order to explore the regulatory mechanism of miR-154-5p involved renal fibrosis, we predicted the target genes of miR-154-5p indicating binding sites between human and rat miR-154-5p sequences and Smad ubiquitination regulatory factor 1 (Smurf1), suggesting that Smurf1 could bind with human and rat miR-154-5p. Here, SMURF1 is linked to renal fibrosis.