The results showed that ACSL4 methylation was inversely correlated with mRNA in most cancers, including BRCA, CESC, COAD, ESCA, GBM, HNSC, KIRP, KIRC, LAML, LIHC, PCPG, PRAD, PEAD, SARC, SKCM, STAD, TGCT, THCA and UCEC. This evidence concerns the gene ACSL4 and cancer.