To further confirm the results of our m6A-seq data, we analyzed gene-specific m6A-IP qPCR assays for several hypermethylated genes (CDCA2, CHAT, TGFA, PLOD2, and EGLN1) and hypomethylated genes (MUC15, HRG, HIF1A, ZNF677, and PAK6), which might participate in RCC development. The gene discussed is CHAT; the disease is renal cell carcinoma.