We have identified a number of Alu RNAs that were edited in HC blood or lung biopsy samples but were unedited in disease cohorts; MS, COV-S, FLU-S, and IBD, synthesized unedited Alu RNAs from DNA templates, and tested their ability to activate IRF and NF-kB transcriptional responses. Here, TRIM63 is linked to irritable bowel syndrome.