In our mouse model of 4T1 breast cancer, PB manifests the same artifact (tumor-induced T cell suppression) when assessing IFNγ+ CD8+ T cells by ICS, while such an “immunosuppression” was not concluded using the IFNγ-eYFP reporter mice by living cell immunostaining and flow cytometric analysis (Figure S5B). This evidence concerns the gene CD8A and breast carcinoma.