To explore the effect of TOPK inhibitor on the proliferation capability of pituitary tumor cells, MMQ cells and GH3 cells were treated using 0.1% DMSO (control group, 0 μmol/L) and different concentrations of HI-TOPK-032 (2 μmol/L, 5 μmol/L, 10 μmol/L), and then, the cell proliferation capability was detected by CCK8 assay. This evidence concerns the gene PBK and pituitary tumor.