In addition, we also detected the expression of apoptosis-related proteins Bax and Bcl-2 by Western Blot assay (Supplementary Figure 2), and found that 5 μmol/L and 10 μmol/L of HI-TOPK-032 significantly up-regulated Bax expression but down-regulated Bcl-2 expression in MMQ cells and GH3 cells, which further validated the induction of HI-TOPK-032 on the apoptosis of pituitary tumor cells. Here, BAX is linked to pituitary tumor.