The over-expression of α9 nAChRs in A2058 and MDA-MB 435 cells also induced mesenchymal-like melanoma cells with the loss of cell-cell adhesion junctions, whereas α9 knockdown in A2058 cells led to an increase in the number of cell-cell adhesion junctions and epithelial-like melanoma cells. This evidence concerns the gene IGKV1D-22 and melanoma.