Our analysis revealed that 19/168 buffers were able to detect predominantly PD-derived α-synuclein aggregation, showing an at least two-fold difference between PD and MSA curves; 109/168 buffers showed no major differences in the main parameters describing the kinetic curve of the RT-QuIC when the MSA aggregation pattern was compared to PD; while 40/168 buffers showed an at least two-fold difference between MSA-derived α-synuclein and PD-derived α-synuclein aggregation. Here, SNCA is linked to Parkinson disease.