By screening the correlation, expression, and prognosis of these 20 genes, we picked out that CDK1, CCNB1and E2F1 were mostly overexpressed in ACC samples compared with normal ones (Fig. 5f, all p < 0.05), and positively relevant with the expression of CENPF (Fig. 5e, all p < 0.01). The gene discussed is CENPF; the disease is adrenal cortex carcinoma.