To confirm the roles of WT1 in tumor growth of BRAFV600E PTC cells, IHH4 cells were treated with lentiviral WT1 shRNA to stably knockdown WT1 expression, and western blotting was performed to measure the WT1 knockdown efficiency in the IHH4 cells (Fig. 7A). This evidence concerns the gene WT1 and neoplasm.