To test whether zinc aspartate would also affect Th1 autoimmunity in vivo, we induced experimental autoimmune encephalitis (EAE), a mouse model for MS, by stimulating 2D2 TCR tg CD4+ T cells with MOG35-55 peptide in vitro, differentiating them into Th1 cells and transferring them into C57BL/6 recipient mice (Fig. 5E). This evidence concerns the gene CD4 and Autoimmunity.