ANXA5 and colorectal carcinoma: Interestingly, while CM of untreated CRC cells had no impact on neutrophil survival, apoptotic tumour cell-derived factors substantially enhanced the proportion of viable neutrophils after 24 h of culture, as determined by annexin V/propidium iodide staining (Fig. 2A) and tetrazolium salt reduction assay (Fig. S3C).