In order to reveal the role of MYC/miR-26a in colon cancer, MYC over-expressing HCT116 and Caco-2 cells were further transfected with miR-26a, which reversed the effect of MYC over-expression as indicated by down-regulated cell number count (HCT116 cells, Fig. 5A; Caco-2 cells, Fig. 5B), cell viability (HCT116 cells, Fig. 5C; Caco-2 cells, Fig. 5D), and clone formation (HCT116 cells, Fig. 5E; Caco-2 cells, Fig. 5F). This evidence concerns the gene MYC and malignant colon neoplasm.