VPS25 and infection: As shown in Figs 1A and S1B, compared with the control group, the levels of viral mRNA were significantly reduced after knockdown of endogenous Tsg101, EAP20 (VPS25), CHMP4B, and CHMP7 in the early entry stage (1h post-infection), indicating that these ESCRT complex subunits play important roles in the early entry stage of CSFV.