Therefore, we conducted colony formation and MTS experiment to determine the effect of knocking down iron metabolism-related genes on cell proliferation ability in different colorectal cancer cell lines, and the result showed that inhibition of SLC48A1 promoted the proliferation of colon cancer cells, and silencing of SLC39A8 expression suppressed the proliferation of colon cancer cells in vitro. These studies also suggest that SLC48A1 and SLC39A8 might be potential predictors of clinical prognosis and therapeutic targets for colon cancer. This evidence concerns the gene SLC48A1 and malignant colon neoplasm.