Six Ψ synthase genes were associated with the prognosis, of which the expression of PUS1, PUS7, RPUSD1 and RPUSD3 was positively associated with the malignancy of glioma (HR > 1), and TRUB1 was negatively associated with the malignant grade (HR < 1) (Figure 3). This evidence concerns the gene RPUSD3 and glioma.