METTL3 and melanoma: To explore the function of METTL3 in melanoma, we silenced the expression of METTL3 in A375 and A875 cells with shRNA transfection, which reduced METTL3 expression by approximately 90% relative to that in non-targeted control (NTC) cells, as confirmed by qRT-PCR and Western blotting (Figure 2A).