BDNF and Alzheimer disease: To validate the effect of autolysosome formation on BDNF nuclear translocation in AD in vitro, mouse hippocampal neuron HT22 cells were treated with the proton pump inhibitor Bafilomycin A1 of different concentrations (10 nM, 30 nM, and 50 nM) for 24 h to inhibit the autolysosome formation.