These results indicated that RGG presented the anti-liver fibrosis activity via PI3K-Akt pathway and others to regulate pyrimidine metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, phenylalanine metabolism, and primary bile acid biosynthesis, and then the mitochondrial structural and function of liver cells may be improved. Here, AKT1 is linked to Hepatic fibrosis.