CP variants were also detected in individuals who did not show signs of ALS at the time of the clinic visit, and there were eleven CP variants within control samples (within ALS2, SETX, OPTN and PFN1), four CP variants in the pre-fALS cohort (within FIG4, OPTN and CHCHD10), three CP variants within individuals with other MNDs (within SQSTM1, OPTN and PFN1) and three CP variants in uncharacterized individuals (within SQSTM1 and SETX; Supplementary Table 8). This evidence concerns the gene ALS2 and amyotrophic lateral sclerosis.