In line with the knockdown data, Gαi2 expression inhibited in a dosage-dependent manner both the Wnt3a-induced signaling in cells with otherwise low pathway activity (Fig. 4g, h and Supplementary Fig. 8h–j) and the basal Wnt signaling in colorectal cancer cell lines with high endogenous pathway activity (Fig. 4i, k). This evidence concerns the gene WNT3A and colorectal cancer.