To investigate whether NTSR to ALS inhibitors is related to CNV, we estimated the copy numbers of CYP450, GST, and ABC transporter genes in the Brazilian E. crus-galli samples lacking ALS target-site mutations and observed no amplification of CYP450 and GST genes but slight expansion of ABC transporter genes (P < 0.05, Student’s t-test) (Supplementary Data 9), indicating the existence of other NTSR mechanisms that are not related to CNV. The gene discussed is ABCG2; the disease is amyotrophic lateral sclerosis.