PRF1 and neoplasm: To investigate whether the expression of LINC01123, B7–H3, or miR-214-3p in HNSCC cells affects CD8+T-cell function, we analyzed the expression of TNF-α, IFN-γ, perforin, and granzyme B in the CD8+T cells by flow cytometry after coculture with the tumor cells (Fig. 4A, B).