Our focus was to address whether chronic hM4Di-DREADD-mediated inhibition of CaMKIIα-positive forebrain excitatory neurons during the early postnatal window alters anxiety-like behavior, and our studies indicate no significant effects of PNCNO administration despite the baseline sex differences noted in anxiety- and despair-like behavior uncovered in male and female cohorts. The gene discussed is CAMK2A; the disease is Anxiety.