GNAI1 and Anxiety: The results of the present study clearly demonstrate that Gi-mediated inhibition of forebrain excitatory neurons using the hM4Di-DREADD during either the postnatal (P2 to P14) or juvenile (P28 to P40) windows does not evoke any significant behavioral change on conflict-based tasks assessing anxiety-like behavior, namely the OFT, EPM test, and LD avoidance test in adulthood.