We then performed in vitro and in vivo assays, which validated that overexpression of IRF-2 in GC cell lines resulted in both the capability of colony formation and cell proliferation as well as the upregulation of AMER-1 protein levels and activation of the Wnt/β-catenin signaling pathway; IRF-2 knockdown resulted in the opposite trend. This evidence concerns the gene AMER1 and gastric cancer.