Based on a mass spectrometric study that found phosphorylation on 12 out of 14 serines in the C‐terminal LCD of TDP‐43 in ALS spinal cord (Kametani et al, 2016), we also mutated these 12 sites (S373, S375, S379, S387, S389, S393, S395, S403, S404, S407, S409 and S410) to create “12D” or “12A” variants (Fig 1D). Here, TARDBP is linked to amyotrophic lateral sclerosis.