In this experiment, the levels of P-PI3K/PI3K, P-AKT/AKT, and P-FoxO1/FoxO1 in the experimental group were higher than those in the control group; the positive expression rate of FoxO1 in CRC was 50%, and its expression was significantly correlated with the depth of tumor invasion, differentiation, and lymph node metastasis, which could provide some reference indicators for the occurrence and development of CRC in clinical practice. The gene discussed is FOXO1; the disease is colorectal carcinoma.