RBPJ and myocardial infarction: Subsequent detection of mRNA expression and protein levels of CYLD in cardiac macrophages by qRT-PCR and Western blot assay revealed that CYLD levels were significantly lower in the MI group and the sh-NC group than those in the sham group, while the sh-RBP-J group presented with partially restored levels of CYLD (all P < 0.01, Figures 4C,D).