This has been demonstrated using both the Ednrb-knockout (Ednrb-KO) transgenic mouse model of Hirschsprung disease (Lane, 1966; Hosoda et al., 1994; Soret et al., 2020), as well as patient samples taken from aganglionic resected gut (Graham et al., 2020) (Figure 5). This evidence concerns the gene EDNRB and Hirschsprung disease.