The Treg-related genes (TGFB1, Foxp3, CTLA-4, IL-10, PRF1, GZMB) were declined in Jurkat T cells and PBMCs cocultured with Agrin-deficient NSCLC cells (Figures 4E, F).Although M1 macrophages and CD8 T cells also played an important role in TIME, no significant alteration of macrophage polarization or CD8 T cells was detected in the THP-1 cells or PBMCs cocultured with Agrin-deficient NSCLC cells (Figures S5B, C). This evidence concerns the gene CD8A and non-small cell lung carcinoma.