In our study, we found that Agrin enhanced NSCLC development and regulated tumor-associated regulatory T cell (Treg) infiltration via enhancing interleukin-6 (IL-6) expression and secretion through PI3K/AKT pathway, suggesting that Agrin acted as an oncogene and augmented Tregs in NSCLC immune microenvironment. The gene discussed is AKT1; the disease is neoplasm.